Effect of Benzylaminopurine and Indole acetic acid in in vitro multiplication of pineapple (Ananas comosus L Merr) cv Smooth cayenne.
DOI:
https://doi.org/10.37376/1571-000-023-001Keywords:
IAA, Ananas comosus, micropropagation.Abstract
AbstractIn vitro multiplication of Smooth cayenne pineapple was tested in agar solidified full strength MS medium containing 6-benzyleaminopurine (BAP) at 2.25, 3.25, 4.25 and 5.25 mg l-1 alone and in combination with indole acetic acid (IAA) at 0.75, 1.75, 2.75 and 3.75 mg l-1. BAP at 2.25 mg l-1 induced the highest shoot formation (9 shoots/ 60 days). Other combinations of BAP and IAA resulted in either less or an equal shoot formation as that of singly applied BAP at 2.25 mg l-1. Statistical analysis showed that the shoot formation was mainly controlled by BAP. IAA, on the other hand, had no direct effect on shoot formation. However, it interacted significantly with BAP. The IAA interaction with BAP could be of positive and negative natures depending on the concentrations of both hormones. Singly applied BAP at 2.25 mg/l was the best for multiplication of secondary explants of Smooth cayenne pineapple.
Downloads
References
References
Almeida W. A., Santana, B., Rodriguez, G.S., and Costa, M.A., 2002. Optimization of a protocol for the micropropagation of pineapple. Rev. Brasil. Fruticul. 24(2): 296-300.
Almeida, W. A. P., Matos, A. P. and Souza, A. D. S. 1997. Effect of benzylaminopurine (BAP) on in vitro proliferation of pineapple (Ananas comosus (L) Merr.). Acta, Hort. 425: 235- 242.
Aydieh A. A., Ibrahim, M. K. H. and Ibrahim, A., 2000. In vitro propagation and fruiting of pineapple. Egyp. J. Hort.27: 289-304
Be L. V. and Debergh, P. C., 2006. Potential low cost micropropagation of pineapple (Ananas comosus). S. Afr. J. Bot. 72: 191- 194.
Bhatia, P. and Ashwath, N., 2002. Development of a rapid method for micropropagation of a new pineapple (Ananas comosus (L) Merr. Clone Yeppoon gold. Acta Hort.575: 125- 131
Bordoloi, N. D. and Sarma, C. M., 1993. Effect of various media composition on in vitro propagation of Ananas comosus (L) Merr. J. plant Sci Research. 9: 50- 53.
Boxus, P., Terzi, J.M. lievens, C.H. Pylyser, M. Ngaboyamabina, P. and Duhem, K., 1991. Improvement and perspectives of micro-propagation techniques applied to some hot climate plant. Acta. Hort. 289; 55-64
Broomes, V.F.A. and McEwan, F. A. 1994. Heat treatment for enhanced responsiveness of dormant axillary buds of pineapple. Turriaba 44(2): 117- 121
Dewald, M. G, More, G. A. Sherman, W. B. and Evans, M. H. 1988. Production of pineapple plants in vitro. Plant Cell Report.7: 535- 537.
Escalona, M., Lorenzo, J. C. Gonzalez, B. Daquinta, M. Gonzalez, J. L. Desjardins, Y. and Borroto, C. G. 1999. Pineapple (Ananas comosus L. Merr) micropropagation in temporary immersion systems. Plant Cell Report.18 (9): 743-748.
Fernando, K., 1986. In vitro propagation of Muritus pineapple. Trop.Agriculturist. 142: 7- 12.
Firoozabady, E. and Gutterson, N. 2003. Cost effective in vitro propagation methods for pineapple. Plant Cell Report. 21; 844- 850.
Fitchet, M. 1990. Clonal propagation of Queen and Smooth cayenne pineapples. Acta. Hort. 275: 261- 266
Hirimburegama K. and Wijesinghe, L. P. J. 1992. In vitro growth of Ananas comosus L.Merr (Pineapple) shoot apices on different media. Acta Hort. 319:203-208.
Khatun M. M., Khanam, D. Hoque, M. A. and Quasem, A. 1997. Clonal propagation of pineapple through tissue culture. Plant Tissue Cult 7(2): 143- 148.
Kofi, O. F and Adachi, T. 1993. Effect of cytokinins on the proliferation of multiple shoots of pineapple in vitro. SABRAO Journal, 1993, 25(1): 59- 69
Liu L. J., Rosa-Marquez, E. and Lazardi, E. 1989. Smooth leaf (spineless) red spanish pineapple (Ananas comosos (L) Merr) propagated in vitro. J. Agr. Univ. Puerto Rico 73: 301- 311.
Mathew, V.H., Rangan, T.S., 1981. Growth and regeneration of plantlets in callus culture of pineapple. Sci. Horti. 14: 227- 234.
Mathew, V.H., Rangan, T.S., 1979. Multiple plantlets in lateral bud and leaf explant in vitro cultures of pineapple. Sci Hortic. 11: 319- 328.
Mathew, V. H., T. S. Rangan and S. Naryanaswamy. 1976. Micropropagation of Ananas sativus in vitro. Z.Phlanzenphysiol. 79: 450- 454
Murashige T and Skoog, F. 1962. A revised medium for rapid growth and bioassay with tobacco tissue. Physiol. Plant. 15: 473- 497
Rahman K. W., Amin, M. N. and Azad, M. A. K. 2001. In vitro rapid clonal propagation of pineapple Ananas comosus (L) Merr. Plant Tiss Cult 11: 47- 53.
Sharrock S. 1992. In vitro propagation of pineapple (Ananas comosus) and plantain (Musa spp.) technical and economic considerations. Proceedings of the Tenth Annual Conference of the Barbados Society of Technologists. pp. 61-69
Singh, D. B. and Manual, A. B. 2000. Assessment of pineapple plants developed from micropropagation instead of conventional suckering. Trop. Sci. 40(4):169-173.
Soneji, J. R., Rao, P. S. and Mhatre, M. 2002a Somaclonal variation in micropropagated dormant axillary buds of pineapple (Ananas-comosus L., Merr.). J. Hort. Sci. Biotech. 77 (1): 28-32.
Soneji, J. R., Rao, P. S. and Mhatre, M. 2002b In-Vitro Regeneration from Leaf Explants of Pineapple (Ananas-Comosus L, Merr). J. Hort Sci Biotech. 2002, Vol 11, Iss 2, pp 117-119
Srinivasa-Rao, N.K; R. Doreswamy and E.K. Chanko. 1981. Differentiation of plantlets in hybrid embryos callus of pineapple. Sci. Hort. 1981. 15: 235- 238
Sripaoraya S., Marchant, R. Power, J. B. and Davey, M. R. 2003. Plant regeneration by somatic embryogenesis and organogenesis in commercial pineapple (Ananas comosus L). In Vitro Cell. Devl. Biol. Plants, 39 (5): 450- 454.
Teixeira, S. L., Ribeeiro, J. M. and Teixeira, M. T. 2006. Influence of NaClO on nutrient medium sterilization and on pineapple (Ananas comosus cv. Smooth cayenne) behavior. Plant Cell Tiss. Org. Cult. 86: 375- 378.
Wakasa, K. 1989. Pineapple (Ananas comosus L. Merr). Biotechnology in Agriculture and Forestry, V. (5), Trees II. pp 13- 29. (Ed. Y.P.S. Bajaj). Springer-Verlang.
Zepeda, C. and Sagawa, Y. 1981. In-vitro propagation of pineapple. Hort. Sci.16 (4); 495
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Journal of Science and Humanities- Faculty of Arts and Science Al – Marj
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
