Sterilization and establishment of pineapple shoot tip
DOI:
https://doi.org/10.37376/1570-000-016-002الكلمات المفتاحية:
BAP; Ananas comosus; Sterilization; Establishment; Contamination; Cloroxالملخص
Out of eight sterilization treatments made of combinations of Mercuric chloride (HgCl2),
Clorox and Ethanol at different concentrations and exposure times, Clorox (25%) for 25
minutes was the best treatment resulting in successful sterilization of 56 % of crown tips of
Smooth cayenne pineapple. The sterilized crown tip grew and produced multiple shoots on
agar solidified full strength MS medium enriched with 6-benzyleaminopurine (BAP) at four
different concentrations (2.25, 3.25, 4.25 and 5.25 mg l-1), but at different shoot formation
rate. After 60 days of incubation, the highest shoot formation (7 shoots / tip) obtained in
medium enriched with BAP at 2.25 mg l-1 followed by BAP at 3.25 mg l-1 (4 shoots). BAP at
4.25 and at 5.25 mg l-1 resulted in formation of 3 and 2 shoots per tip. The low percentage of
successfull sterilization of crown tips (56 %) indicated that elimination of microbial
ccontamination is still a serios problem and main drawback that prevent full investigation
and optimization of establishment of crown tips of pineapple. Nevertheless, the few
successfully sterilized crown tip proliferated into shoots good enough to investigate the next
multiplication stage.
التنزيلات
التنزيلات
منشور
كيفية الاقتباس
إصدار
القسم
الرخصة
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