Effect of medium states and pH on in vitro shoot formation and growth of pineapple (Ananas comosus L. Merr) cv Moris

الملخص

Abstract

In vitro shoots formation, shoot weight and length of Moris pineapple explants cultured on full strength MS medium enriched with sucrose at 20 g/ l, 6-Benzylaminopurine (BAP) at 2.0 mg/l and solidified with agar at 4 and 8 g/l were compared with semi submerged explants in static liquid medium under four different medium pH adjustments (5.0; 5.5; 6.0 and 6.5). The highest shoot formations (7 shoots/ explant) was obtained on liquid medium adjusted to pH 5.0 and the heaviest (0.36 g) and tallest (61 mm) shoots obtained also in liquid medium but adjusted to pH 6.0 . However, the superiority of liquid medium could not be maintained if the medium were adjusted to inappropriate pH. No difference in shoot formation between liquid and solid medium both adjusted to pH 6.0 (4 shoots) and between liquid media adjusted to pH 5.5 and 6.5 and solid media adjusted to pH 5.0 (6 shoots). At pH 5.0; 5.5 and 6.5 liquid and solid media resulted in equal shoot weight and shoot length. Different growth parameters have different optimal pH adjustments of medium and valid comparison of states of medium could not be claimed if only one pH adjustment was made.