The full length of gene (x14K) that differentially expressed with retinoic acid (RA) treated Xenopus laevis embryos
Keywords:
Retinoic acid, Xenopus embryos, x14K-geneAbstract
The differential display RT-PCR technique has allowed me to isolate a gene whose expression is upregulated by retinoic acid in stage 14 x.laevis embryos. The M-band pattern which are generated from this strategy ( probe paper), was appeared to be induced by retinoic acid. This M-CDNA fragment was used as a probe. The full length cDNA clone encoding region of this probe-fragment, termed x14K-gene was isolated from a kidney xenopus cDNA library.
Sequencing of x14K-gene generated a continuous sequence of 2271 bp that contained an open reading frame of 1284 bp. Expression of x14K-gene in rabbit reticulocyte lysate produced a protein of the predicted size (47 kD) as determined from the largest open reading frame. A search of the GenBank database has identified that x14K-gene is more closely related to a Xenopus mitotic phosphoprotein MP43 than any other previously identified proteins. The two Xenopus proteins share 84% identity. Most lamins (lamin B1, A, C & C2) which were observed to be substrates of a family of cyclin-dependent kinases (CDKs), apppeared to have homology to the carboxy end of x14K-gene.
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Aida Halilagic, Maiia H. Zile, and Michele Studer (2003). A novel role for retinoids in patterning the avian forebrain during presomite stages. Development 130, 2039-2050.
Amanda Janesick, Jason Shiotsugu, Mao Taketani, and Bruce Blumberg ( 2012). RIPPLY3 is a retinoic acid-inducible repressor required for setting the borders of the pre-placodal ectoderm. Development 139, 1213-1224.
Brennan H. C., Niijar S. and Jones E. A. (1999). The specification and growth factor inducibility of the pronephric glomus in Xenopus laevis. Development 126, 5847-5856.
Coleman, T. R., Tang, Z., and Dunphy, W. G. (1993). Negative regulation of the weel protein kinase by direct action of the niml/cdr1 mitotic inducer. Cell 72, 919-929.
Birnboim, H. C. and Dolly, J. (1979). A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acid Res. 7, 1513-1523.
Draetta, G. Luca, F., Westendorf, J., Brizuela, L., Ruderman, J., Beach, D. (1989). Cdc2 protein kinase is complexed with both cyclin A and B:evidence for proteolytic inactivation of MPF. Cell 56, 829-838.
Durston, A. J., Timmermans, J. P. M., Hage, W. J., Hendriks, N. J. de Vries, Heideveld, M. and Nieuwkoop, P. D. (1989). Retinoic acid causes an anteroposterior transformation in the developing central nervous system. Nature 340, 140-144.
Feinberg, A. P. and Vogelstein, R. (1984). A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 137, 717-722.
Hanks, S.K. (1987). Homology probing: identification of cDNA clones encoding members of the protein serine kinase family. Proc. Natl. Acad. Sci. USA 84, 388-392
Hellmich, M. R., Pant, H. C., Wada, E. and Battey, J. F. (1992). Neuronal cdc2-like kinase: Acdc2-related protein kinase with predominantly neuronal expression. Proc. Natl. Acad. Sci. USA 89, 10867-10871.
Hocevar, B. A., Burns, D. J. and Fields, A. P. (1993). Identification of protein kinase C (PKC) Phosphorylation sites on Human Lamin B. J. Biol. Chem. 268, 7545-7552.
Hunt, T., Luca, F. C. and Ruderman, J. V. (1992). The requirements for protein synthesis and degradation, and control of destruction of cyclins A and B in the meiotic and mitotic cell cycles of the clam embryo. J. Cell Biol. 116, 707-724.
Isabel Olivera-Martinez and Kate G. Storey (2007). Wnt signals provide a timing mechanism for the FGF-retinoid differentiation switch during vertebrate body axis extension. Development 134, 2125-2135.
Izumi, T. and Maller, J. L. (1993). Elimination of cdc2 phosphorylation sites in the cdc25 phosphatase blocks initiation of M-phase. Mol. Biol. Cell 4, 1337-1350.
Johana M. Lampert, Jocchen Holzschuh, Susanne Hessel, Wolfgang Driever, Klaus Vogt and Johannes von Lintig (2003). Provitamin A conversion to retinal via the B, B-carotene-15,15'-oxygenase (bcox) is essential for pattern formation and differentiation during zebrafish embryogenesis. Development 130, 2173-2186.
Jonk, L. J. C., de Jonge, M. E. J., Kruyt, F. A. E., Mummery, C. L., van der Saag, P. T., and Kruijer, W. (1992). Aggregation and cell cycle dependent retinoic acid receptor mRNA expression in P19 embryonal carcinoma cells. Mech. Dev. 36, 165-172.
King, R. W. Jackson, P. K., Kirschner, M. W. (1994). Mitosis in Cransition. Cell. 79, 563-571.
Krieg, P. A. and Melton, D. A. (1984). Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs. Nucleic Acids Res. 12, 7057-7070.
LaRosa, G. J. and Gudas, L. J. (1988b). Early retinoic acid-induced F9 teratocarcinoma stem cell gene ERA-1: Alternate splicing creates transcripts for a homeobox-containing protein and one lacking the homeobox. Mol. Cell. Biol. 8, 3906-3917.
Lee, S. W., Tomasetto, C. and Sager, R. (1991). Positive Selection of candidate tumor-suppressor genes by subtractive hybridization. Proc. Natl. Acad. Sci. USA 88, 2825-2829.
Liang, P., and Pardee, A. B. (1992). Differential Display of Eukaryotic Messenger RNA by Means of the Polymerase Chain Reaction. Science 257, 967-971.
Lupas, A., Van Dyke, M. and Stock, J. (1991). Predicting Coiled coils from Protein sequences. Science 252, 1162-1164.
Maden, M. (1985). Retinoids and the control of pattern in limb development and regeneration. TIG 1, 103-107
Maniatis, T., Fritsch, E. F., and Sambrook, J. (1982). Molecular cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.
Mason, C., Lake, M., Nebreda, A. and Old, R. (1996). A novel MAP kinase phosphatase is localised in the branchial arch region and tail tip of Xenopus embryos and is inducible by retinoic acid. Mech. Dev. 55, 133-144.
Meyerson, M., Enders, G. H., Wu, C. L., SU, L. K., Gorka, C., Nelson, C., Harlow, E. and Tsai, L. H. (1992). A family of cdc2-related protein kinases. EMBO J. 11, 2909-2917
Minshull, J., Golsteyn, R., Hill, C. S., Hunt, T. (1990). The A- and B-type cyclin associated cdc2 kinases in Xenopus turn on and off at different times in the cell cycle. EMBO J. 9, 2865-2875.
Paris, J., LeGuellec, R., Couturier, A., LeGellec, K., Omilli, F., Camonis, J., MacNeill, S. and Philippe, M. (1991). Cloning by differential screening of a Xenopus cDNA coding for a protein highly homologous to cdc2. Proc. Natl. Acad. Sci. USA 88, 1039-1043.
Paris, J. and Philippe, M. (1990). Poly (A) metabolism and polysomal recruitment of maternal mRNAs during early Xenopus development. Dev. Biol. 140, 221-224.
Patschinsky, T., Hunter, T., ESch, F. S., Cooper, J. A. and Sefton, B. M. (1982). Analysis of the sequence of amino acids surrounding sites of tyrosine phosphorylation. Proc. Natl. Acad. Sci. USA 79, 973-977.
Ronald R. Waclaw, Bei Wang, and Kenneth Campbell (2004). The homeobox gene Gsh2 is required for retinoid production in the embryonic mouse telencephalon. Development131, 4013-4020.
Ruiz i Altaba, A. and Jessell, T. (1991a). Retinoic acid modifies mesodermal patterning in early Xenopus embryos. Genes & Dev. 5, 175-187.
Schuuring, E., van Deemter, L., Roelink, H., and Nusse, R. (1989). Transient expression of the proto-oncogene int-1 during differentiation of P19 embryonal carcinoma cells. Mol. Cell. Biol. 9, 1357-1361.
Sive, H. L., Draper, B. W., Harland, R. M. and Weintraub, H. (1990). Identification of a retinoic acid-sensitive period during primary axis formation in Xenopus laevis. Genes Dev. 4, 932-942.
Stukenberg, P. T., Lustig, K. D., McGarry, T. J., King, R. W., Kuang, J. and Kirschner, M. W. (1997). Systematic identification of mitotic phosphoproteins. Current Biology 7, 338-348.
Summerbell, D. and Harvey, F. (1983). Vitamin A and the control of pattern in developing limbs. Prog. Clin. Biol. Res. 110A, 109-118
Tickle, C., Alberts, B.M., Wolpert, L. and Lee, J. (1982). Local application of retinoic acid to the limb bud mimics the action of the polarising region. Nature 296, 564-565
Wei, L. N., Blaner, W. S., Goodman, D. S. and Nguyen-Huu, M. C. (1989). Regulation of the cellular retinoid binding proteins and their messenger ribonucleic acids during P19 embryonal carcinoma cell differentiation induced by retinoic acid. Mol. Endocrinol. 3, 454-463.
Wendling O., Dennefeld C., Chambon P. and Mark M. (2000). Retinoid signaling is essential for patterning the endoderm of the third and fourth pharyngeal arches. Development 127, 1553-1562.
Wright, C. V. E., Cho, K. W. Y., Hardwicke, J., Collins, R. H. and DeRobertis, E. M. (1989). Interference with function of a homeobox gene in Xenopus embryos produces malformations of the anterior spinal cord. Cell 59, 81-93.
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