دراسة سيرولوجية باستخدام البكتيروفاج في الكشف عن البكتيريا Pectobacterium carotovorum في أنسجة البصل المصابة والتربة المحيطة بها

Abstract

The study was done to detect the bacteria that cause soft rot on onions which called (Pectobacterium carotovorum) This was done by using bacteriophage, where the bacteria were isolated from the infected onion and the bacteriophage was isolated from the moist soil surrounding the infected onion to test the efficacy of Bacteriophage against these bacteria. A syrological test was done for bacteriophage by inject it  on NewZealander   white rabbit. Bacteriophage was detected in the infected tissues of onions, and it was also possible to distinguish it from a group of viruses by ELISA test. It was found that the associated bacteriophage with Pectobacterium carotovorum was effective against these bacteria in the laboratory food environment. And it was found that its possible to product antibiotics in the rabbit's body after inject it with bacteriophage through serological tests. It is also possible to detect bacteriophage associated with bacteria in onion tissues, as well as in the surrounding moist soil.